The Zeiss 510 NLO located in 251-B LSA has the following components:

• Zeiss inverted Axiovert200M microscope with manual XY-stage.
• Spectra-Physics MaiTai HP laser for 2-photon Imaging (690nm - 1020nm)
• Spectra-Physics Pulse Picker and Frequency Doubler
• Becker-Hickl SPC-830 Time-Correlated Single Photon Counting board for FLIM
• Hamamatsu MCP-PMT for 2-photon FLIM
• PMC-100 high speed detector for confocal FLIM
• 3 Confocal Channels
• 1 non-descanned detection (NDD) channel
• Laser lines: 458nm, 488nm, 514nm, 543nm, 633nm
• Objectives Available:
• Plan-Neofluar 10x/0.30 WD=5.6mm air
• Plan-Neofluar 25x/0.50 WD=2.0mm imm (multi-immersion)
• Plan- Neofluar 40x/1.3 oil WD=0.17mm
• Plan-Apochromat 63x/1.4 oil
• Plan-Apochromat 100x/1.4 oil, WD=0.1mm
• Warner TC-324B Heater Controller
• Up to 2048 x 2048 pixel resolution
• Up to 40x Zoom
• Speed ~ 1 frame/second (Zoom 1, 512 x 512)
• Live cell imaging
• Time-series
• Z-series
• X,Y,Z,T series
• FRET: Foerster Resonance Energy Transfer
• ROI scanning
• FRAP: Fluorescent recovery after photobleaching
• FLIM: Fluorescent Lifetime Imaging Microscopy
• 128 x 128 pixel resolution
• 1-channel 2p non-descanned detection
• 1-channel 1p fiber-out detection
• frequency doubler light (440nm-500nm)
• Physiology Triggering