ZEISS LSM 510 META/NLO AXIOIMAGER with MAITAI HP

The Zeiss LSM 510/NLO META located in the MIC-Annex (126-LSA) has the following components: Zeiss upright AxioImager microscope with manual XY-stage

• Spectra-Physics MaiTai HP laser for 2-photon Imaging (710nm - 1020nm)
• 3 Confocal Channels, including 1 spectral detection channel (META)
• 2-channel non-descanned detection (NDD)
• Laser lines: 458nm, 488nm, 514nm, 543nm, 633nm
• Specially designed projection system for retina-imaging
• Objectives Available:

• Plan-Neofluar 10x/0.30 WD=5.6mm air
• Achroplan 20x/0.5 W WD=1.9mm dipping objective
• Achroplan IR 40x/0.80 W WD=3.6mm dipping objective
• Achroplan IR 63x/0.95 W WD=2.0mm dipping objective

• Up to 2048 x 2048 pixel resolution
• Up to 40x Zoom
• Speed ~ 1 frame/second (Zoom 1, 512 x 512)
• Up to 8 colors simultaneously
• Spectral emission profiling and separation (linear unmixing)
• Live cell imaging
• Time-series
• Z-series
• X,Y,Z,T, lamda series
• FRET: Foerster Resonance Energy Transfer
• ROI scanning
• FRAP: Fluorescent recovery after photobleaching
• Excitation Profiling
• Physiology Triggering
• Physiology Set-up